Skip to main content
. 2019 Dec 17;41(5):650–660. doi: 10.1038/s41401-019-0328-3

Fig. 2. ASM inhibition attenuated leptin resistance in Ob-Rb-silenced RAECs.

Fig. 2

Representative Western blot gels for Ob-Rb and summarized data showing the efficiency of Ob-Rb siRNA transfection (a). RAECs were transfected with Ob-Rb siRNA for 48 h and were treated with AMI (10 μM) or IMI (10 μM) for 24 h. Then, 100 nM leptin was added for 15 min prior to the collection of the cells. The protein expression of SOCS3 (b) and the ratio of p-STAT3/STAT3 (c) were tested using Western blotting. The data are the means ± SEMs from three experiments. *P < 0.05 vs. vehicle control (Vehl Scr); #P < 0.05 vs. the Ob-Rb siRNA alone-transfected group.