Table 3.

Cd and Zn content in ECT tissue and in ECT culture media

	Cd (ng/ECT)	Zn (ng/ECT)	Cd (ng/40 μL media)	Zn (ng/40 μL media)
Ctl (n = 3)	0	37.56 ± 0.70	0.05 ± 0.01	13.28 ± 0.78
Cd 20 μM (n = 3)	66.70 ± 2.09	36.05 ± 3.20	76.35 ± 1.15	9.98 ± 1.19
Zn 50 μM (n = 3)	0	67.78 ± 0.87	0.06 ± 0.01	196.68 ± 2.18
Cd 20 + Zn 50 μM (n = 3)	56.44 ± 1.3**	75.10 ± 2.66*	88.37 ± 1.56**	191.84 ± 1.57

As expected, Cd content was only noted in treated ECTs. Zn was present in Ctrl ECTs and increased after Zn treatment. ECT Cd content decreased and media Cd content increased following Zn and Cd cotreatment. *P < 0.05 vs. corresponding 50 µM Zn group, **P < 0.01 vs. corresponding 20 µM Cd group. n = 3 ECTs per group