Fig. 2.

Effect of PDGF-BB on the expression of TSPO protein and mRNA in VSMCs. a The effect of PDGF-BB on TSPO expression in A10 cells. A10 cells were incubated with the indicated concentrations of PDGF-BB for 24 h. TSPO expression was determined by WB (n = 6). b The effect of PDGF-BB on TSPO expression in A10 cells. A10 cells were incubated with PDGF-BB (10 ng/mL) for the indicated times (n = 6). c–e The role of PKC/MAPK signaling in PDGF-BB-induced TSPO expression in A10 cells. c, d The expression levels of TSPO protein and mRNA were assessed by WB and PCR after cotreatment with PDGF-BB (10 ng/mL) and PKCI (10³ nM), MAPKI (10² nM), PKAI (10² nM) or Ca²⁺I (10³ nM) for 24 h (n = 6; *P < 0.05 vs. control group; ^#P < 0.05 vs. PDGF-BB alone). e A10 cells were treated with the indicated concentrations of PMA (1, 10, 10², 10³, 10⁴ nM), a PKC agonist, for 24 h. TSPO expression was assessed by WB. The results are expressed as the percent change compared with the control (n = 8; *P < 0.05 vs. control group)