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. 2020 Aug 13;23(9):101453. doi: 10.1016/j.isci.2020.101453

Figure 1.

Figure 1

U87 GBM Cells Oxidize Fatty Acids and Ketone Bodies to Support Growth

(A) Relative mRNA expression of the three CPT1 isoforms in GBM based on analysis of the TCGA dataset from patient-derived GBM tumors. N = 4 non-tumor, N = 156 GBM. See also Figure S1.

(B) Quantification of the effects of 100 mM ETOM treatment and shRNA targeting CPT1A on U87 cell growth. Culture growth was assessed following a 7 day treatment with ETOM administered twice per week. n = 3 replicates. Error bars indicate ± SD. Univariate generalized linear model F(2,5) = 47.37, p = 5.6x10-4, post-hoc Tukey HSD (∗∗ = p<0.01; ∗∗∗ = p<0.001).

(C and D). Effects of FA (C) and ketone (D) supplementation under variable concentrations of glucose following a 7 day growth period in U87 cells. n = 3 replicates. For all univariate generalized linear models for different concentrations of supplements within each glucose concentration p<0.01. Post-hoc Tukey HSD significance is reported for each supplement concentration against its control. Error bars denote ± SD. (∗ = p<0.05; ∗∗ = p<0.01; ∗∗∗ = p<0.001).