Figure 2.

U87 GBM Cells Oxidize Fatty Acids to Generate TCA Cycle Intermediates and Currency Metabolites

(A and B) Effects of ETOM and CPT1A knockdown in U87 cells on fractional contribution (the percent contribution of carbons from radiolabeled palmitate) and relative amounts of relevant TCA cycle intermediates and currency metabolites using ¹³C-palmitate LC-MS analysis. Glucose concentration = 2.5 mM. n = 3 replicates. Error bars = ± SD (∗ = p < 0.05, ∗∗ = p < 0.01, ∗∗∗ = p < 0.001).

See also Figure S2.