Figure 5.

GBM Cells Oxidize FAs to Generate Acetyl-CoA for Use within the TCA Cycle

(A and B) LC-MS analysis of ¹³C-palmitic acid fractional contribution following treatment with 100 μM ETOM. (A) Heatmap showing effects of ETOM on fractional contribution across metabolites. Relative expression is measured as standard deviations from the group mean. (B) Quantification of the effects of ETOM on intracellular carbon labeling of acetyl-CoA, palmitic acid, and other relevant metabolites associated with the TCA cycle.

(C) Effects of ETOM on relative amounts of relevant metabolites as in (B). ETOM concentration used: 100 μM. n = 3 replicates. Error bars = ± SD (∗ = p < 0.05, ∗∗ = p < 0.01, ∗∗∗ = p < 0.001).