Figure 6.

IDH1 Mutant GBM Cells Express Higher Levels of CPT1A and Utilize Fatty Acids to Produce 2-HG

(A) mRNA expression of CPT1 isoforms in IDH1 mutant (N = 5) and WT (N = 62) patient-derived GBM cultures based on analysis of microarray data.

(B) Western blot of CPT1A and CPT1C across a panel of IDH WT and IDH1 mutant lines. Error bars = ± SD (∗ = p < 0.05, Kruskal-Wallis).

(C and D) Analysis of the effects of ETOM (100 μM) in an IDH1 mutant cell line on fractional contribution and relative amounts of relevant metabolites using ¹³C-palmitic acid LC-MS. n = 3 replicates. Error bars = ± SD (∗ = p < 0.05, ∗∗ = p < 0.01, ∗∗∗ = p < 0.001).

See also Figure S5.