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. 2020 Aug 21;8:763. doi: 10.3389/fcell.2020.00763

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Copyright © 2020 Pejhan, Del Bigio and Rastegar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Results of protein studies by Western blot and ELISA in different regions of Rett Syndrome (RTT) and control brain tissues. (A) Western blot results for MeCP2 isoforms quantified and analyzed in four brain regions (frontal cerebrum, hippocampus, amygdala, and cerebellum) for mean of controls show non-significant variations between the regions. (B) Western blot results for MeCP2 isoforms quantified and analyzed in four brain regions for mean of RTTs show higher level of MeCP2E1 isoform in the cerebellum compared to the frontal cerebrum or hippocampus. (C) Mean of MeCP2 isoforms in RTT and controls are not significantly different in relative RTT/control analysis. (D) Western blot results for ProBDNF quantified and analyzed in four brain regions for mean of controls and RTTs show non-significant inter-regional variations without significant difference between RTT and controls. (E) ELISA results for BDNF quantified and analyzed in four brain regions for mean of controls and RTTs show significantly higher levels in Hippocampus compared to frontal cerebrum and cerebellum of the controls. (F) Mean of ProBDNF and BDNF in RTT and controls are not significantly different in relative RTT/Control analysis. Frontal cerebrum (Fc); Hippocampus (Hp); Amygdala (Am); Cerebellum (Cb); N = 3 ± Standard Error of the Mean (SEM); One-way ANOVA (ELISA)/Kruskal-Wallis test (WB) with multiple pairwise comparisons used among different brain regions. Student’s t-test (ELISA)/Mann-Whiney test (WB) was used to compare controls and RTT samples for each parameter. The significance level was determined at P < 0.05 (#).