Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 3;10:14598. doi: 10.1038/s41598-020-71548-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Development of human peripheral blood mononuclear cells in the human Skin and Immune System-humanized NSG mouse model. (A) Representative flow cytometry analysis of human immune cell (hCD45+) reconstitution in peripheral blood mononuclear cells (PBMCs) of human Skin and Immune System-humanized NSG mice at 10–12 weeks post-transplantation demonstrates high levels (> 10%) of human immune cells in the blood. (B) Quantification of human immune cell reconstitution (n = 22; 3 independent experiments) in PBMCs of hSIS-humanized mice at 10 weeks post-transplantation. (C) Representative flow cytometry analysis of human PBMCs (hCD45+ PBMCs) in human Skin and Immune System-humanized mice at 10–12 weeks post-transplantation demonstrates readily detectable levels of various human immune cell types (B cells-hCD19+hCD3- human PBMCs, αβ T cells-hCD3+ human PBMCs, hCD4+ T cells, hCD8+ T cells, hγδTCR+ T cells- hγδ TCR+ CD3+ human PBMCs, natural killer cells (NK)-hCD57+ hCD3- human PBMCs, monocytes (hMo)-hCD14+ CD3- human PBMCs, and granulocytes (hPMN)-hCD66b+ hCD3- human PBMCs). (D) Quantification of human immune cell subtypes (n = 4) in human PBMCs (hCD45+ PBMCs) of human Skin and Immune System-humanized mice at 10–12 weeks post-transplantation.