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Expression of glycolytic pathway proteins in TEC from WT and PFKFB2 KI mice. (A–F) Primary cultures of tubular epithelial cells (TEC) and fibroblasts (Fib) were established and cell lysates prepared. Western blots showed that expression of the major proteins involved in glycolysis were unchanged in the cells from PFKFB2 KI mice compared to WT. Protein loading was determined by GAPDH. Expression of PFKFB2 was reduced in the PFKFB2 KI cells. ***p < 0.001. Mean + S.D.
