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. 2020 Sep 3;10:14531. doi: 10.1038/s41598-020-71475-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The glycolytic and mitochondrial function of mouse kidney epithelial cells from wild type and PFKFB2 KI mice was measured using the XFe96 Seahorse analyser. (A,B) There was reduced glycolysis in glucose-stimulated cells and reduced glycolytic capacity in PFKFB2 KI TEC compared to WT. (C, D) Mitochondrial respiration was not significantly different. *p < 0.05, ***p < 0.001. Mean + S.E.M.