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. 2020 Sep 3;10:14584. doi: 10.1038/s41598-020-71128-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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GRN overexpression (O/E) rescued proliferation and neutralized radiosensitivity enhanced by miR-107 mimic in PC-3 cells. Relative expression of GRN mRNA (a) and protein (b) after cotransfection with NC mimic + pcDNA3.1, miR-107 mimic + pcDNA3.1, NC mimic + pcDNA3.1-GRN, or miR-107 mimic + pcDNA3.1-GRN, respectively. (c) Cell proliferation and (d) colony formation of PC-3 cells cotransfected with the above RNAs and plasmids after IR. Data were representative of more than three independent experiments, with each performed in triplicate. (*p < 0.05; **p < 0.01). pcDNA3.1 represents the empty vector pcDNA™3.1⁽⁻⁾. pcDNA3.1-GRN represents the vector pcDNA™3.1⁽⁻⁾ containing the GRN gene construct. NS represents the none significant.