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. 2020 Sep 3;10:14584. doi: 10.1038/s41598-020-71128-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MiR-107 and shGRN altered radiation-induced cell cycle distribution and delayed apoptosis by repressing p21 expression and CHK2 phosphorylation in PC-3 cells. (a) Cell cycle distribution in PC-3 cells transfected with miR-107 mimic or negative control (NC), and each group was treated with 0 or 8 Gy IR. The bar graphs show the relative G1/S and G2/S ratios between the NC- and miR-107-transfected cells at 24 h. (b) Apoptosis was assayed by flow cytometry at 7 days after irradiation with 8 Gy. The graph indicates the percentage of apoptotic cells based on annexin-V-FITC and PI staining. (c) Cell cycle distribution in PC-3 cells transfected with shGRN or PCDNA3.1 (scramble), and each group was treated with 0 or 8 Gy IR. (d) Apoptosis was assayed at 7 days after irradiation with 8 Gy by annexin-V-FITC and PI staining. (e) Overexpression of miR-107 (left) or knockdown of GRN by shGRN (right) reduced p21 expression and CHK2 phosphorylation (Thr68) in response to IR at 72 h. Data were representative of more than three independent experiments, with each performed in triplicate. (*p < 0.05; **p < 0.01; ***p < 0.001).