Table 1.
Bacterial strains and plasmids
|
Strains and plasmids |
Genotype or description |
Reference or source |
|---|---|---|
|
E. coli |
||
|
DH5αλ pir |
φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 deoR _λpir |
[31] |
|
S17-1 |
recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 λ-pir |
[32] |
|
P. fluorescens |
||
|
Pf0-1 |
Wild-type, Apr |
[26] |
|
Pf0-1ΔalgB |
ΔalgB (bases 49462–50781 removed from Pf0-1) |
This study |
|
Pf0-1ΔalgD |
ΔalgD (bases 1119622–1120929 removed from Pf0-1) |
This study |
|
Pf0-1Δalg44 |
Δalg44 (bases 1116871–1117999 removed from Pf0-1) |
This study |
|
Plasmids |
||
|
pGEM-T Easy |
Apr; cloning vector for PCR products |
Promega |
|
pSR47s |
Kmr; sacB-containing suicide vector (requiring R6K replication origin) |
[33] |
Apr, ampicillin resistance;Kmr, kanamycin resistance.