Fig. 2.

PAI resolves interspecies HB differences in rat and mouse. (a) Absorption spectra of HB extracted from lysed whole blood of mouse (purple) and rat (pink). (b) Interspecies differences in ${\mathrm{THb}}^{\text{MSOT}}$ obtained from PAI measurements of female mouse ($n=15$) and rat ($n=14$) blood show a significantly higher value in rat blood, which was underscored by differences in biochemical HB (c) and HCT (d). Notably, the RBC value (e) was found to be lower in the rat, but with higher mean corpuscular hemoglobin (MCH) per red blood cell (f). While the mean corpuscular hemoglobin concentration (MCHC) was comparable between the species (g), the mean corpuscular volume MCV (h) was higher in the rat. Data are represented as $\mathrm{mean}\pm \mathrm{SEM}$, significance *** $p<0.0001$ by unpaired $t$-test.