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. 2020 Sep 3;10:14543. doi: 10.1038/s41598-020-71429-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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ΔT_m values for the F21T_TERRA (0.4 μM) determined by FRET upon addition of 0.4, 0.8, and 1.6 μM metallohelices. The buffer conditions were 10 mM potassium phosphate (pH 7) and 10 mM KCl. Data shown are expressed as the mean of three independent experiments; error bars indicate the standard error of the mean. Symbols (*, **, ***) and (ns) at the top of the bars indicate statistically significant difference (p ≤ 0.1, p ≤ 0.05, p ≤ 0.01) and statistically insignificant difference (p > 0.1), respectively.