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. 2020 Aug 26;20(5):17. doi: 10.3892/etm.2020.9144

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Dynamic changes of Deptor in the progression of renal interstitial fibrosis by immunohistochemistry. Deptor expression in kidneys of rats in the (A) sham group and in rats after (B) 3, (C) 7 and (D) 14 days after UUO. Magnification, x400 (E) Statistical analysis of Deptor immunohistochemistry positive area. n=6. (F) Protein expression level of Deptor in the kidneys of rats was analyzed by western blotting. (G) Statistical analysis of Deptor expression. n=3. (H) Correlation analysis of Deptor and collagen levels. Sirius-Red positive area of collagen and Deptor immunohistochemistry positive area were plotted and analyzed using Pearson's correlation coefficient analysis. Magnification x400. All data are presented as the mean ± SEM. ^*P<0.05, ^**P<0.01 vs. sham group. UUO, unilateral ureteral obstruction; Deptor, DEP domain-containing mTOR interacting protein; IOD, integral optical density.