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. 2020 Sep 3;10:14523. doi: 10.1038/s41598-020-71316-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The insertion of the Achilles tendon (AT) into the calcaneus (CAL) is depicted on plastinates and in histology. (A) Plastinated slice. Black arrows: demarcated trabeculae; dotted box: corresponds to an area similar to the dotted box in B; 1–3: fibrous septae, similar to the ones in B. Scale bar: 4 mm, (B) Masson–Goldner stain. Black box, median fibrous septum; PT, paratenon; 1–3: fibrous septae, similar to the ones in A; white arrow: median calcaneal prominence; yellow dotted line: demarcation between AT and CAL. Scale bar: 1 mm, (C) H&E stain. AC, adipocytes (located in bone marrow); black arrows: border between AT and CAL in greater detail compared to B; blue arrows: collagen bundles; white asterisk: folding artefact; yellow arrows: calcaneal trabeculae. Scale bar: 200 μm; a, anterior; l, lateral; m, medial; p, posterior.