Skip to main content
. 2020 Aug 21;8:987. doi: 10.3389/fbioe.2020.00987

TABLE 1.

Treatments of the differentiated MDMs.

Sample Description 0 to 24 h 24 to 48 h
Untreated cells cRPMI (3 mL) cRPMI not changed
Negative control cRPMI (2.5 mL) Vehicle (0.5 mL)
LPS cRPMI (2.5 mL) with LPS (1 μg/mL) Vehicle (0.5 mL)
LPS + MP10 cRPMI (2.5 mL) with LPS (1 μg/mL) MP (0.5 mL of 0.06 mM stock in cRPMI). Final concentration 0.01 mM
MP control cRPMI (2.5 mL) MP (0.5 mL of 0.06 mM stock in cRPMI). Final concentration 0.01 mM
IL-4+IL-13 (M2 control) cRPMI with IL-4 and IL-13 (20 ng/mL) Treatment not changed
Triton X-100 (positive control for LDH) cRPMI (3 mL) Triton X-100 (0.3 mL). Final concentration 0.2% (v/v)

Vehicle is a solution of absolute ethanol (0.005% in cRPMI; v/v).