TABLE 1.
Sample Description | 0 to 24 h | 24 to 48 h |
Untreated cells | cRPMI (3 mL) | cRPMI not changed |
Negative control | cRPMI (2.5 mL) | Vehicle (0.5 mL) |
LPS | cRPMI (2.5 mL) with LPS (1 μg/mL) | Vehicle (0.5 mL) |
LPS + MP10 | cRPMI (2.5 mL) with LPS (1 μg/mL) | MP (0.5 mL of 0.06 mM stock in cRPMI). Final concentration 0.01 mM |
MP control | cRPMI (2.5 mL) | MP (0.5 mL of 0.06 mM stock in cRPMI). Final concentration 0.01 mM |
IL-4+IL-13 (M2 control) | cRPMI with IL-4 and IL-13 (20 ng/mL) | Treatment not changed |
Triton X-100 (positive control for LDH) | cRPMI (3 mL) | Triton X-100 (0.3 mL). Final concentration 0.2% (v/v) |
Vehicle is a solution of absolute ethanol (0.005% in cRPMI; v/v).