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Characterization of induced pluripotent stem cell (hiPSC)s-derived neural stem cells (NSCs) lines 1 and 2: RT-PCR analysis of NSCs markers PAX6, NESTIN, SOX1, SOX2, the negative control OCT4 and the housekeeping gene ACTIN; confocal imaging of NSCs immunolabeled for the neural progenitor markers Pax6 and Nestin. NSC Lines 1 and 2 are shown at 60× magnification. RT: Reverse transcriptase.
