Figure 4.

Effect of WNV, USUV and ZIKV infection on NSC viability. Cell viability was measured by MTT assay at 4 and 7 days post infection with WNV lineage 2, USUV Europe 1 and ZIKV Asian lineage at MOI 0.1 and MOI 1. Analysis was performed in eight replicates and repeated in three independent experiments. Data represent mean values ± SD of all experiments. WNV vs. USUV and vs. ZIKV, * p < 0.05, Student’s t-test.