Table 1.
Oligonucleotide primers for RT-PCR analysis of cell differentiation markers.
| Target Gene | Forward/Reverse Primers (5′–3′) | References | |
|---|---|---|---|
| Neural stem cells markers | NESTIN | GAAGGTGAAGGGCAAATCTG CCTCTTCTTCCCATATTTCCTG |
[40] |
| PAX6 | TCTAATCGAAGGGCCAAATG TGTGAGGGCTGTGTCTGTTC |
[41] | |
| SOX1 | GCGGAAAGCGTTTTCTTTG TAATCTGACTTCTCCTCCC |
[42] | |
| SOX2 | TTGTCGGAGACGGAGAAGCG TGACCACCGAACCCATGGAG |
[42] | |
| Pluripotency marker | OCT4 | GTGGAGGAAGCTGACAACAA CAGGTTTTCTTTCCCTAGCT |
[43] |
| Housekeeping gene | ACTIN | GGACTTCGAGCAAGAGATGG AGCACTGTGTTGGCGTACAG |
[44] |