Table 2.
Primers and probes used for qRT-PCR analysis mRNA levels of genes involved in innate antiviral response.
| Target Gene | Forward/Reverse Primers/TaqMan Probe (5′–3′) | Reference |
|---|---|---|
| RIG1 | ACCAGAGCACTTGTGGACGCT TGCCGGGAGGGTCATTCCTGT |
[45] |
| MDA5 | CAGAAGGAAGTGTCAGCTGCTTAG TGCTGCCACATTCTCTTCATCT |
[46] |
| CGAS | CCTGCTGTAACACTTCTTAT TTAGTCGTAGTTGCTTCCT |
[47] |
| TLR3 | GAAAGGCTAGCAGTCATCCA CATCGGGTACCTGAGTCAAC |
[48] |
| TLR7 | CTTGGCACCTCTCATGCTCT GTCTGTGCAGTCCACGATCA |
[49] |
| TLR8 | AGTTTCTCTTCTCGGCCACC GGAACATGTTTTCCATGTTTCTGT |
[49] |
| TLR2 | GGCCAGCAAATTACCTGTGTG AGGCGGACATCCTGAACCT FAM-CCATCCCATGTGCGTGG-MGB |
[50] |
| MAVS | AGCAAGAGACCAGGATCGACTG CGCAATGAAGTACTCCACCCA |
[46] |
| NFKB | GCCAACAGATGGCCCATACC TGCTGGTCCCACATAGTTGC |
[51] |
| IRF3 | AGCAGAGGACCGGAGCAA AGAGGTGTCTGGCTGGGAAA FAM-ACCCTCACGACCCACATAAAATCTACGAGTTTG-TAMRA |
[52] |
| IRF7 | TACCATCTACCTGGGCTTCG AGGGTTCCAGCTTCACCA |
[46] |
| TNFA | CCAGACCAAGGTCAACCTCC CCCTCCCAGATAGATGGGCT |
[53] |
| IFNA1 | AGAATCTCTCCTTTCTCCTG TCTGACAACCTCCCAGGCAC |
[54] |
| IFNB1 | GAGCTACAACTTGCTTGGATTCC CAAGCCTCCCATTCAATTGC FAM-ACAAAGAAGCAGCAATTTTCAGTGTCAGAAGCT-TAMRA |
[52] |
| IFNL1 | GAGGCCCCCAAAAAGGAGTC AGGTTCCCATCGGCCACATA |
[49] |
| IFNL2 | AATTGTGTTGCCAGTGGGGA GCGACTGGGTGGCAATAAAT |
[49] |
| IFNL3 | AGGGCCAAAGATGCCTTAG CAGCTCAGCCTCCAAAGC |
[49] |
| IFIT1 | TCTCAGAGGAGCCTGGCTAA TGACATCTCAATTGCTCCAG |
[49] |
| IFIT2 | AAGAGTGCAGCTGCCTGAA GGCATTTTAGTTGCCGTAGG |
[49] |
| RSAD2 | CTTTGTGCTGCCCCTTGAG TCCATACCAGCTTCCTTAAGCAA |
[55] |
| CASP1 | AAGACCCGAGCTTTGATTGACTC AAATCTCTGCCGACTTTTGTTTCC |
[56] |
| CASP3 | TGCATACTCCACAGCACCTG TTCTGTTGCCACCTTTCGGT |
[57] |
| IL1B | GAGCAACAAGTGGTGTTCTCC AACACGCAGGACAGGTACAG |
[58] |
| GAPDH | GAAGGTGAAGGTCGGAGTC GAAGATGGTGATGGGATTTC FAM-CAAGCTTCCCGTTCTCAGCC-TAMRA |
[59] |