Figure 1.

Effect of dihydroorotate dehydrogenase (DHODH) inhibitors on lymphocytic choriomeningitis virus (LCMV) multiplication. (A–F) Effect on LCMV propagation (green line) and cell viability (blue line) of the indicated compounds. A549 cells were treated with serial dilutions of each compound starting 2 h prior to infection (multiplicity of infection [MOI] = 0.01) with clone 13 (CL-13) expressing Zoanthus sp. green fluorescent protein (ZsG) (rCL-13/ZsG). Levels of ZsG expression were determined at 48 h pi (four replicates). Cell viability (six replicates) was evaluated using the CellTiter-Glo assay after 48 h of treatment. Data were normalized with respect to the vehicle control group. Error bars represent mean +/− SEM. (G) CC₅₀, EC₅₀, and selectivity index (SI = CC₅₀/EC₅₀) values for recombinant LCMV (rLCMV), Lassa virus (rLASV), and r3Can.