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. 2020 Aug 11;12(8):878. doi: 10.3390/v12080878

Figure 1.

Figure 1

The resting and activated states of the phage p2 baseplate. (A) Negative-staining 3D reconstruction of phage p2 virion tail tip and baseplate (EMD-1699, [6]). The map is contoured at 2.5σ. (B) CryoEM 3D reconstruction phage p2 purified and cross-linked baseplate (EMD-1706 [6]). The map is contoured at 2.5σ. (C) Crystal structure of phage p2 purified baseplate in complex with VHH5 (PDB ID 2WZP, [6]). VHH5 molecules are not shown for clarity. (D,E) Central section of the 3D reconstructions shown in panels A and B with the baseplate crystal structure (shown in panel C) fitted in the maps. The green/black dots indicate unassigned EM densities/EM density rings. (F) The crystal structure of phage p2 purified baseplate in activated conformation, with the receptor-binding domains pointing in the opposite direction to the capsid, is shown for comparison (PDB ID 2X53 [6]).