Table 1.
Primers utilized for RT-qPCR.
| Gene | Sequence | Accession | Efficiency |
|---|---|---|---|
| 18S | FW: GAACGAGACTCTGGGCATGC | NR_036642 | 102% |
| REV: CTGAACGCCACTTGTCCCTC | |||
| DDX58 | FW: GAGCACTGGTGGATGCCTTA | XM_024996055.1 | 157% |
| REV: GCTGTCTCTGTTGGTTCGGA | |||
| IRF7 | FW: GCCTCCTGGAAAACCAACTT | NM_001105040.1 | 127% |
| REV: CCTTATGAGGGTCGGTAGGGG | |||
| NFKB | FW: CGAGGTTCGGTTCTACGAGG | NM_001102101.1 | 134% |
| REV: TGCAGGAACACGGGTTACAGG | |||
| IFNB | FW: TCCAGCACATCTTCGGCATT | NM_174350.1 | 104% |
| REV: TTCCCTAGGTGGGGAACGAT | |||
| ISG15 | FW: GGTATCCGAGCTGAAGCAGTT | NM_174366 | 115% |
| REV: ACCTCCCTGCTGTCAAGGT | |||
| STAT4 | FW: TTCTTCCCATGTCGCCAAGT | NM_001083692.2 | 104% |
| REV: AACCAGATGTGATTGTTGGCA | |||
| IFI30 | FW: GCATGCAGCTCTTGCACATC | NM_001101251.2 | 131% |
| REV: GGCCCCAAGAGTTCTTACCC | |||
| PSMB9 | FW: ATCTACCTGGCCACCATCAC | NM_001034388 | 115% |
| REV: AGGAGAGTCCGAGGAAGGAG | |||
| PSMB8 | FW: ACTGGAAGGCAGCACAGAGT | NM_001040480 | 124% |
| REV: ATTGTGCTTAGTGGGGCATC | |||
| B2M | FW: AFTAAGCCGCAGTGGAGGT | AC_000167.1 | 123% |
| Rev: CGCAAAACACCCTGAAGACT | |||
| CXCL10 | FW: ACACCGAGGCACTACGTTCT | CB533091 | 121% |
| Rev: TAAGCCCAGAGCTGGAAAGA | |||
| CXCL16 | FW: CTTGTGAGGGCAGATTGTGA | CK770974 | 105% |
| Rev:GGTCAATAGCTGGTTAGTTGTGAA | |||
| CD4 | FW: GGGCAGAACGGATGTCTCAA | NM_001103225.1 | 110% |
| REV: ATAGGTCTTCTGGAGCCGGT | |||
| CD8a | FW: TACATCTGGGCTCCCTTGGT | NM_174015.1 | 130% |
| REV: CCACAGGCCTGGGACATTTG | |||
| CD8b | FW: AGCTGAGTGTGTTGATGTTCT | NM_001105344.2 | 93% |
| REV: TTCTGAGTCACCTGGGTTGG | |||
| CD79b | FW: TGATTCCCGGGCTCAACAAC | XM_002696068.6 | 160% |
| REV: CTGCCAGATCCGGGAACAAG |
DDX58: DExD/H-Box Helicase 58; IRF7: Interferon Regulatory Factor 7; NFKB: Nuclear Factor Kappa-Light-Chain-Enhancer of Activated B Cells; IFNB: Interferon Beta; ISG15: Interferon Stimulated Gene 15; STAT4: Signal Transducer and Activator of Transcription 4; IFI30: IFI30 Lysosomal Thiol Reductase; PSMB9: Proteasome 20S Subunit Beta 9; PSMB8: Proteasome 20S Subunit Beta 8; B2M: Beta-2-Microglobulin; CXCL10: C-X-C Motif Chemokine Ligand 10; CXCL: C-X-C Motif Chemokine Ligand 16. FW: Forward primer; REV: Reverse Primer.