Fig. 4.
Inhibition of the ρ1 wild-type receptor by combinations of neurosteroids. Sample current responses to 0.4–0.8 μM GABA (Popen ∼0.3) followed by a coappplication of GABA + 10 μM 3α5βP + 20 μM 3α5βPS (A), GABA + 10 μM 3α5βP + 20 μM β-estradiol (B), or GABA + 20 μM 3α5βPS + 20 μM β-estradiol (C). The bars above the traces indicate the durations of applications of the drugs. The dashed and dotted lines show theoretical current levels simulated by the models assuming same or distinct binding sites, respectively, for the paired steroids.