TABLE 2.
Analysis of modulation of the ρ1 receptor by steroids
The table summarizes the results of fitting the steroid conc.-Popen response data from ρ1 wild-type and I307Q mutant receptors to eq. 2. The experiments were conducted in the presence of GABA, and L was calculated as (1−Popen,GABA)/Popen,GABA. The number of binding sites for steroids was constrained to five, and the maximal Popen for GABA was assumed to be 0.999.
| Receptor | Steroid | KC (μM) | c |
|---|---|---|---|
| ρ1 wild-type | 3α5βP | 2.85 ± 0.62 | 1.25 ± 0.02 |
| 3α5βPS | 51.1 ± 25.3 | 2.34 ± 0.89 | |
| β-Estradiol | 16.4 ± 4.8 | 1.47 ± 0.07 | |
| 5αTHDOC | 38.2 ± 28.6 | 0.58 ± 0.07 | |
| ρ1(I307Q) | 3α5βP | 20.5 ± 15.1 | 0.55 ± 0.05 |
| 5αTHDOC | 28.6 ± 26.1 | 0.49 ± 0.08 |