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. 2020 Aug 6;12(8):856. doi: 10.3390/v12080856

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Mutational analysis of the intervening sequence (IS). (A) Deletions made in the 32 nucleotide long IS region (Δ14, Δ24, Δ29). Adenylate residues are highlighted in yellow in the wt sequence. (B) In vitro translation analysis of the IS mutants in panel A. (C and D) Northern blot analysis of protoplast infections with deletion mutants in panel A at (C) 22 °C and (D) 29 °C. (E) Substitutions in the IS are shown in red (341, 343, 345) and adenylate residues are highlighted in yellow in the wt sequence. (F) In vitro translation analysis of IS mutants in panel E. (G and H) Northern blot analysis of protoplast infections with deletion mutants in panel E at (G) 22 °C and (H) 29 °C.