Table 2.
The sequences of primers used for real-time PCR.
| Gene | F Primer Sequence (5′ to 3′) | R Primer Sequence (5′ to 3′) | Concentration (μM) | Amplicon Sizes (bp) |
|---|---|---|---|---|
| H-STAT1 | CAGACCACAGACAACCTGCT | ACAGAGCCCACTATCCGAGA | 10 | 112 |
| P-STAT1 | GGGCTCTGCTAAAGGACCAG | AGTAAGGTTCGCCTCCGTTC | 10 | 120 |
| F-STAT1 | GGGAACCTTACTTCCACGCA | TCAGCCGCCATGACTTTGTA | 10 | 100 |
| H-ISG15 | GCGCAGATCACCCAGAAGAT | GTTCGTCGCATTTGTCCACC | 10 | 167 |
| P-ISG15 | CTATGAGGTCTGGCTGACGC | GGCTTGAGGTCATACTCCCC | 10 | 147 |
| F-ISG15 | GCAGATCGCCCAGAAAACTG | GACCCTTGTGGTTCCTCACC | 10 | 185 |
| H-E2F2 | TGCCCAGCTACTGCTACCTA | ATCCCCTCCAGATCCAGCTT | 10 | 154 |
| P-E2F2 | GTACCCGCAGACTATGCCTC | AATGCACTTCCCCTTGGGAG | 10 | 187 |
| F-E2F2 | GATCAGTTTCTCCCCGCCTT | AGTTAATCAGCAGGTCCCCG | 10 | 113 |
| H-CDKN1A | ATGTGGACCTGTCACTGTCT | CGTTTGGAGTGGTAGAAATCTGTC | 10 | 189 |
| P-CDKN1A | ATGACCTGGGAGGGGGC | GCACAAGGGTACAAGACAGC | 10 | 105 |
| F-CDKN1A | GGAGCGATGGAACTTCGACT | GGGAGTGAGGCATGAGAGTG | 10 | 232 |
| H-IRF9 | ACCAGGATGCTGCCTTCTTC | CCTGGTGGCAGCAACTGATA | 10 | 268 |
| P-IRF9 | CCCTGCCATCTGGAAGACTC | CCCATTGGTCTCTGCCAACT | 10 | 289 |
| F-IRF9 | TTCTTCAAGGCATGGGCGAT | TTTGTGGTACCCGCATCCTC | 10 | 278 |
| H-GAPDH | CAAATTCCATGGCACCGTCA | GACTCCACGACGTACTCAGC | 10 | 211 |
| P-GAPDH | TCGGAGTGAACGGATTTGGC | TCTCGCTCCTGGAAGATGGT | 10 | 226 |
| F-GAPDH | AAGGTCGGTGTGAACGGATT | TTTGCCGTGGGTGGAATCAT | 10 | 152 |