Table 1.
Reaction conditions affect the number and density of CLPs.
| Sample | Ratio | % Sucrose in Peak CLP Fraction * | Normalized Relative CLPs ** |
|---|---|---|---|
| WT + DNA Cargo | 1:1 | 18.68–20.00 | 0.59 |
| WT + M38 | 0.5:1 | 14.59 | 0.26 |
| 1:1 | 14.40 | 0.56 | |
| 2:1 | 14.63 | 0.73 | |
| WT + (M38 + DNA Cargo) | 0.5:1:1 | 17.62 | 0.17 |
| 1:1:1 | 18.93 | 0.40 | |
| 2:1:1 | 18.65 | 1.00 | |
| (WT + DNA Cargo) + M38 | 1:1:1 | 18.94 | 0.42 |
| (WT + M38) + DNA Cargo | 1:1:1 | 17.27 | 0.43 |
* Peak fractions are the fractions determined to have the most CP signal from densitometry analysis. The % sucrose for those fractions was determined by measuring the refractive index using a refractometer and converting the value to percent sucrose. ** The total number of CLPs was determined by DLS in biological triplicates. The values were averaged and then normalized throughout all samples. The values provided in both columns are an average of at least three biological replicates, except for WT + DNA Cargo sucrose percentages (2 replicates).