Table 2.
MICs1, MBCs2, HC503 and TI4 values of brevinin-1OS and its analogues determined for the six different microorganisms.
| Microorganisms | MICs 1/MBCs 2 (µM) | |||||||
|---|---|---|---|---|---|---|---|---|
| Brevinin-1OS | OSa | OSb | OSc | OSd | OSe | OSf | ||
| Gram-positive | S. aureus | 4/16 | >512/>512 | 256/>512 | 256/>512 | 16/32 | 4/4 | 8/16 |
| MRSA | 32/32 | >512/>512 | >512/>512 | >512/>512 | 32/32 | 8/8 | 16/64 | |
| E. faecalis | 8/16 | >512/>512 | >512/>512 | >512/>512 | 32/64 | 16/16 | 32/64 | |
| Yeast | C. albicans | 8/64 | >512/>512 | 512/>512 | 256/>512 | 16/32 | 4/4 | 8/16 |
| Gam-negative | E. coli | 128/>512 | >512/>512 | >512/>512 | >512/>512 | 32/32 | 16/16 | 8/16 |
| P. aeruginosa | 256/>512 | >512/>512 | >512/>512 | >512/>512 | 64/64 | 16/16 | 64/64 | |
| HC50 3 | 145.8 | >512 | 381.4 | >512 | >512 | 64.03 | 328.3 | |
| TI 4 (Overall) | 5.74 | 1 | 0.53 | 1.59 | 35.92 | 7.13 | 20.52 | |
| TI (Gram-positive and yeast) | 15.33 | 1 | 0.63 | 2 | 45.24 | 9.51 | 21.61 | |
1 MICs is minimal inhibitory concentrations; 2 MBCs is minimal bactericidal concentrations; 3 HC50 is the concentration of the peptides causing 50% haemolysis of the red blood cells. When no 50% haemolysis was observed, at 512 µM, a value of 1024 µM was used to calculate the therapeutic index; 4 Therapeutic index (TI) is the ratio of the HC50 to the geometric mean of MIC. Larger values indicate greater cell selectivity.