Figure 5.

Effect of targeted mutation of genes encoding amino acid transport systems on the toxicity of BMAA. The wild type (WT) of Anabaena and seven mutants inactivating one or two genes involved in amino acid transport were incubated in BG11 or BG11₀ with increasing concentration of BMAA. For each mutant, the corresponding affected transport system (N-I, N-II; N-III, or Bgt) is indicated in parenthesis. All cultures were started with a similar OD at 0.07, under illumination and shaking, and imaged after 12 days of incubation.