Figure 4.
Crocetin reduced the expression of inflammatory cytokines and pro-apoptotic mediators in the livers of DENV-infected mice. BALB/c mice were infected with DENV-2 (4 × 105 FFU/mL) and then treated with crocetin (50 mg/kg) dissolved in 2% DMSO. DENV-infected 2% DMSO-treated and mock-infected 2% DMSO-treated groups of mice were used as positive and negative controls, respectively. RNA samples prepared from liver tissues were converted to cDNA. RT-PCR was conducted, and the Ct values were normalized to that of GAPDH. The mRNA expressions of (A) TNF-α, TRAIL, IL-6 and IL-10, and (B) Fas and FasL, are shown. The black star represents, those groups were compared and found to be significant.