Figure 6.
Crocetin increased HO-1 expression, but reduced COX-2 expression in the livers of DENV-infected mice. BALB/c mice were infected with DENV-2 (4 × 105 FFU/mL) and then treated with crocetin (50 mg/kg) dissolved in 2% DMSO. DENV-infected 2% DMSO-treated and mock-infected 2% DMSO-treated groups of mice were used as positive and negative controls, respectively. RNA samples prepared from liver tissues were converted to cDNA. RT-PCR was conducted, and the Ct values were normalized to that of GAPDH. For the protein expressions, Western blot analysis was conducted with protein samples obtained from liver homogenates, and the blots obtained were quantitated by ImageJ densitometry analysis. The result shows (A) mRNA expressions and (B) protein expressions of HO-1 and COX-2, and (C) represents the densitometry analysis of the immunoblots. The black star represents, those groups were compared and found to be significant.