Figure 1. Screening of phosphorylation expression patterns in PCa cells by western blotting analysis. (A) Protein expression profiling in PC-3 and PC-3M cells by western blotting analysis. Ten micrograms of protein were loaded into lanes 1-4. Lanes 1-2: duplicate loading of protein extracts from PC-3 cells, lanes 3-4: duplication loading from PC-3M cells. (B) Phosphorylation validation in PC-3 and PC-3M cells, by western blotting analysis. Phosphorylation patterns for the amino acids serine (Ser), threonine (Thr), tyrosine (Tyr), and histidine (His) were detected using corresponding antibodies.
