Figure 2. MS-based quantitative proteomic profiling of phosphorylation in PCa. (A) Experimental scheme for quantitative proteomic analysis. PC- 3 (K4R6) and PC-3M (K0R0) were lysed and mixed at equal protein amounts, and tryptic digestion was performed. Phosphorylated peptides were affinity purified using a TiO2 column, and eluted peptides were injected into the nano-LC-MS/MS system. MS spectra were searched in MaxQuant (version 1.5). (B) Overview of the frequency of differentially expressed phosphorylation from proteomics analysis. (C) Correlation of technically replication of MS data.
