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. 2020 Aug 21;7:484. doi: 10.3389/fvets.2020.00484

Figure 1.

Figure 1

pUL46 as a nucleocytoplasmic shuttling protein. (A) cNLS Mapper prediction of the NLSs of pUL46 (in red). (B) NLStradamus prediction of the NLSs of pUL46 (in green squares). (C) NetNES prediction of the NESs of pUL46 (the peaks of lines). (D) Vero cells were transfected with UL46-EGFP, pEGFP-C3, and UL46-N-EGFP; HeLa cells, UL46-EGFP; and NIH/3T3 cells, UL46-FLAG. After 24 h of transfection, Vero, HeLa, and NIH/3T3 cells were subjected to immunofluorescence analysis. Scale bars = 20 μm.