Figure 5.

Precise identification of the NLS of UL46. (A) A schematic representation of deletion mutants aa1–20, aa21–696, aa3–18, aa3–17, and aa4–18 fused with the C-terminus of EGFP. (B) Localization of aa1–20, aa21–696, aa3–18, aa3–17, and aa4–18 mutants; NLS-UL35-FLAG; and UL35-FLAG. All cell nuclei were stained with DAPI. Scale bars = 20 μm. (C) Schematic representations of point mutations in the NLS. (D) Subcellular localization of proteins harboring point mutations in their NLSs. Scale bars = 20 μm.