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. 2020 Aug 21;11:1220. doi: 10.3389/fphar.2020.01220

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Copyright © 2020 Ling, Alattar, Tan, Shah, Ali, Alshaman, Koh and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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After 1 week of acclimatization, randomly selected male Sprague-Dawley were divided into the following groups: (1) Vehicle-treated sham rats; (2) MCAO rats; (3) Pre-melatonin administration to rats undergoing MCAO; (4) Pre-melatonin administration to sham rats. Melatonin or vehicle was administered as a single dose (5 mg/kg), all rats brain was isolated for further biochemical and immunohistochemical analyses.