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. 2020 Aug 21;11:1220. doi: 10.3389/fphar.2020.01220

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Copyright © 2020 Ling, Alattar, Tan, Shah, Ali, Alshaman, Koh and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The immunoblot results of Nrf2, HO-1, and Trx in the cortical and striatum tissue of rat ipsilateral brain. The immunoblot bands were quantified using ImageJ software, and the statistical differences are indicated in the relative graphs. The data are expressed as the mean ± SEM for n = 6 rats/group, and the number of experiments = 3. Data were analyzed by two-way ANOVA followed by post-hoc Bonferroni Multiple Comparison test using graph-pad prism-5 software β-Actin was taken as a loading control. Significance = ^∗∗∗p<0.00, ^∗∗ or ^##p<0.01, and ^# or ^∗p<0.05. The brain tissue for Western blot was collected after 24 h of vehicle or melatonin treatment and stored at −80°C until used, Nrf2, nuclear factor erythroid 2-related factor 2; HO-1, heme oxygenase-1; Trx, thioredoxin.