Figure 7.

Effect of melatonin (5 mg/kg) on the p-NF-κB/Trx pathways (A, B) p-NFκB and Trx were colocalized by double immunofluorescence. Scale bar = 20 μm and (n=5/group). p-NFκB showed higher expression level accompanied with Trx down expression in the MCAO group. p-NF-κB and Trx were visualized by FITC and TRITC, respectively. Significance = * and ** shows significant difference relative to sham operated vehicle group and its value are p < 0.05, and p < 0.01. ^## shows significant difference relative to MCAO operated group and its value is p < 0.01. Significance = ^# or *p < 0.05; and ** or ^##p < 0.01. FITC, fluorescein isothiocyanate; TRITC, tetramethylrhodamine.