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. 2020 Aug 21;11:1313. doi: 10.3389/fphar.2020.01313

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Copyright © 2020 Yamaguchi, Shibata, Ohashi, Enomoto, Ogawa, Otaka, Hiramatsu-Ito, Masutomi, Kawanishi, Murohara and Ouchi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CTRP9 promotes network formation and migration of cultured endothelial cells. (A) Endothelial cell network formation after stimulation with CTRP9 or vehicle. After 24 h of serum deprivation, human umbilical vein endothelial cells (HUVECs) were treated with recombinant CTRP9 (10 μg/ml), or PBS (control) followed by culture in Matrigel-coated dishes. Representative photomicrographs of HUVEC network formation is shown (upper panel). Quantitative analyses of the network area of tubes are shown (lower panel) (n = 3 independent culture experiments). (B) Endothelial cell migration after stimulation with CTRP9 or vehicle. A modified Boyden chamber assay was performed using HUVECs. HUVECs were treated with CTRP9 (10 µg/ml), or PBS (control). Results are presented as mean ± SD. Results are expressed relative to the values compared with control (n = 8 independent assays).