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. 2020 Aug 21;11:1301. doi: 10.3389/fphar.2020.01301

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Copyright © 2020 Kumar, Kaur, Pandit, Tuli, Sak, Jain and Kaur

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of Obeth from O. bracteata on DNA protection against oxidative damage caused by Fenton’s reagent (FR) in DNA nicking assay. (A) Relative mobility of different forms of plasmid DNA exposed to Obeth extract in electrophoresis. Lane 1: Negative control (pBR322 DNA + distilled water); Lane 2: Positive control (pBR322 DNA + FR); Lane 3: pBR322 DNA + FR + 100 µg/mL rutin; Lanes 4, 5, 6: pBR322 DNA + FR + 25, 50, 100 µg/mL Obeth fraction respectively. (B) Densitometric analysis showing relative percentage of different DNA forms in plasmid after treatment with various concentrations of Obeth fraction in the form of table and (C) graph respectively (software used = Lab Image). CN = Control, FR=Fenton reagent.