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. 2020 Aug 21;11:1301. doi: 10.3389/fphar.2020.01301

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Copyright © 2020 Kumar, Kaur, Pandit, Tuli, Sak, Jain and Kaur

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression level p53, NFκB, and COX-2 protein in liver homogenate of Wistar rats detected using Western blotting. (A) Control group (tap water ad libitum) (GI), (B) CCl₄: Olive oil (1:1; 1mL kg^-1b.wt.) induced elevation in the expression level of p53 was noted. (C) Silymarin (100 mg kg^-1b.wt.) + CCl₄, (D) low dose (50 mg kg^-1b.wt.) + CCl₄, (E) medium dose (100 mg kg^-1b.wt.) + CCl₄, (F) High dose (200 mg kg^-1b.wt.) + CCl_4, (G) Negative (only extract 100 mg kg^-1 b/wt.). Histograms showing densitometric analysis of p53, NFκB, and COX-2 protein bands using western blotting. Band density was measured and normalized to that of β-actin. Values are expressed as mean ± SE at level of significance p ≤ 0.05. Data labels with different letters represent significant difference among them.