TABLE 1.

Dataset description.

	Data Seta,b	DNA Extraction	Sequencing	Bioinformatic Processing	Sample Count	Min Depth	Max Depth	ASV/OTUc Count
Unpooled	MMESP	MM	MM	MM	59	1,029	32,305	400
	UTESP	UT	UT	UT	64	1,022	167,922	757
	UTESMMP	UT	UT	MM	58	1,125	114,877	396
	UTEMMSP	UT	MM	MM	61	2,745	33,415	403
	UMESP	UM	UM	UM	64	5,480	273,886	289
	UMESMMP	UM	UM	MM	62	1,963	103,781	418
	UMEMMSP	UM	MM	MM	60	1,445	24,413	411
Pooled	PE	All	MM	MM
	PS	All	All	MM
	PP	All	All	All

^aE, Extraction; S, Sequencing; P, Bioinformatic Processing. ^bPE, pooled post-extraction; PS, pooled post-sequencing; PP, pooled post-bioinformatic processing. All pooled data sets consist of samples randomly drawn from unpooled data sets. ^cASV, Amplicon Sequence Variant; OTU, Operational Taxonomic Unit.