Fig. 7. (A) Circos diagram comparing vif module 5 (vif-M5), jesQS-M9 and jesQS-M18. Module sequences with domain annotations are represented by circle segments as indicated. Regions of extended nucleotide identity are indicated as colored ribbons (red: >90% identity, orange: 80–90% identity according to BLAST). The triplicate A domain, more precisely the A–T didomain, is highly identical in all three modules as shown by broad red ribbons. An entire C–A didomain (or even a T–C–A–T multi-domain) was transferred between jesQS-M18 and vif-M5 as shown by broad orange ribbon. Thin ribbons represent conserved motifs. (B) Phylogenetic network analysis of repeated Ser-activating A domains. The central split mirrors phylogenetic separation of the “triplicate” jesQS domains from the “duplicate” jesMWU domains. The slightly closer relationship of jesQS-A9 and vif-A5 compared to jesQS-A18 is detectable as well. However, the reticulate structure in the middle of the network points towards conflicting phylogenetic signals suggesting further recombination events. The scale bar indicates uncorrected pairwise distances.
