Table 1.
The optimization of key parameters for the preparation of ICA for ZEN.
| Parameter | Concentration | Volume | Solvent |
|---|---|---|---|
| GAM-IgG | 13.1 mg/mL | 0.5 μL | 10 mmol/L PBS (containing 0.15 mol/L NaCl, pH 7.4) |
| ZEN-antigen | 8.8 mg/mL | 1.0 μL | 10 mmol/L PBS (containing 0.15 mol/L NaCl, pH 7.4) |
| ZEN-McAb | 1.0 mg/mL | 3.0 μL | 10 mmol/L PB |
| BSA | 10% | 100 μL | Purified water |
| K2CO3 | 0.2 mol/L | 2.3 μL | Purified water |
Note: The concentrations and volumes of ZEN-McAb and K2CO3 in this table are used in 1 mL GNPs. Other parameters were used for preparing a 30 cm of NC membrane.