Figure 2.

Purification of cdg14a by high-performance liquid chromatography (HPLC) using C18 semi-preparative and analytical columns. The elution profiles show two different wavelengths used: 220 nm (black) and 280 nm (grey), with the elution gradient indicated by a blue line. (A) Chromatogram of the crude venom extract using a C18 semi-preparative column with linear gradient ranging from 6% to 60% solvent B (90% acetonitrile with 0.1% trifluoroacetic acid) at 0.9% solvent B/min, followed by 60% to 100% at 1.3% solvent B/min. The peak of the bioactive fraction is indicated by an arrow. (B) Chromatogram of the bioactive fraction reinjected in the HPLC using a C18 analytical column run at a shallower linear gradient ranging from 23% to 27% solvent B at 0.15% solvent B/min. The peak of the subfraction containing the bioactive peptide cdg14a is indicated by an arrow. (C) Chromatogram of the purified peptide cdg14a, showing a single, symmetrical peak.