Figure 10.

Sequence alignment of Ves a 1 catalytic and auxiliary sites with other lipases: the Ves a 1 sequence from V.affinis (PA11_VESAF) was aligned with the other five phospholipase A₁ (PA1_VESBA, PA1_VESCR, PA1_VESVE, VEST1_VESTR, and PA11_DOLMA8) and five mammalian lipases (PLA1A_HUMAN, LIPC_HUMAN, LIPC_RAT, LIPR2_CAVPO, and LIPL_HUMAN). The corresponding Uniprot KB accession code is listed before the sequence name; for example, P0DMB4|PA11_VESAF indicates a Ves a 1 sequence for which the Uniprot KB accession code is P0DMB4. The green and light blue labeled sites indicate a catalytic site and an auxiliary site, respectively. The red N showed the Asn211 position which was responsible for binding a phospholipid molecule at an auxiliary site. The asterisk (*) and colon (:) point out the identical and conserved regions among species.